[1]陈思思,吴宇,朱馨艺,等.ITGB2促进胶质瘤细胞增殖并受E2F2调控[J].中国临床神经外科杂志,2024,29(08):478-482494.[doi:10.13798/j.issn.1009-153X.2024.08.008]
　CHEN Si-si,ZHU Xin-yi,WU Yu,et al.ITGB2 promotes the proliferation of glioma cells and is regulated by E2F2[J].,2024,29(08):478-482494.[doi:10.13798/j.issn.1009-153X.2024.08.008]
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ITGB2促进胶质瘤细胞增殖并受E2F2调控()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
29
期数:
2024年08期
页码:
478-482494
栏目:
实验研究
出版日期:
2024-08-30

文章信息/Info

Title:
ITGB2 promotes the proliferation of glioma cells and is regulated by E2F2
文章编号:
1009-153X（2024）08-0478-05
作者:
陈思思; 吴宇; 朱馨艺; 邓钢
430060武汉，武汉大学人民医院神经外科（陈思思、吴宇、朱馨艺、邓钢），整形美容外科（陈思思）
Author(s):
CHEN Si-si1; 2;  ZHU Xin-yi1;  WU Yu1;  DENG Gang1
1. Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan 430060, China; 2. Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan 430060, China
关键词:
胶质瘤; 整合素亚单位β2（ITGB2）; E2F2; 细胞增殖; 细胞周期
Keywords:
Glioma;  Integrin subunit β2 (ITGB2);  E2F2;  Cell proliferation;  Cell cycle
分类号:
R 739.41
DOI:
10.13798/j.issn.1009-153X.2024.08.008
文献标志码:
A
摘要:
目的 探讨整合素亚单位β2（ITGB2）在胶质瘤中的表达、功能及其调控机制。方法 收集2018年6月至2019年6月手术切除的25例胶质瘤组织样本以及其邻近的非肿瘤脑组织样本，采用实时定量PCR检测ITGB2 mRNA的表达。体外培养正常人小胶质细胞系（HMC3）和胶质瘤细胞系（A172、T98G、U138-MG），转染E2F2、ITGB2小干扰RNA调控细胞基因表达，CCK-8法和EdU染色法检测细胞增殖，利用流式细胞术检测细胞周期分布，双荧光素酶报告基因实验验证E2F2与ITGB2启动子区域的结合关系，利用免疫印迹法检测E2F2对ITGB2蛋白表达的影响。结果 与瘤周非肿瘤脑组织相比，胶质瘤组织ITGB2 mRNA表达明显上调。敲低ITGB2表达抑制体外培养胶质瘤细胞的增殖，并诱导细胞发生周期阻滞。E2F2结合于ITGB2启动子区域，过表达E2F2促进ITGB2蛋白表达，敲低E2F2抑制ITGB2蛋白表达。过表达E2F2逆转敲低ITGB2对胶质瘤细胞生长的抑制作用。结论 ITGB2在胶质瘤细胞中高表达，且促进胶质瘤细胞增殖，其高表达受E2F2调控。
Abstract:
Objective To explore the expression, function and regulatory mechanism of integrin subunit beta 2 (ITGB2) in glioma. Methods The expressions of ITGB2 mRNA in glioma tissue and adjacent non-tumor brain tissue samples which were collected from 25 patients with glioma undergoing surgical resection from June 2018 to June 2019 was detected by real-time quantitative PCR. Glioma cell lines (A172, T98G, U138-MG) were cultured in vitro. E2F2 and ITGB2 small interfering RNAs were transfected to regulate the gene expression of cells. Cell proliferation was detected by CCK-8 assay and EdU staining, and cell cycle distribution was detected by flow cytometry. The binding relationship between E2F2 and the promoter region of ITGB2 was verified by dual luciferase reporter gene assay, and the influence of E2F2 on the expression of ITGB2 protein was detected by Western blotting. Results Compared with the non-tumor brain tissues adjacent to the tumor, the expression of ITGB2 mRNA in glioma tissues was significantly upregulated. Knockdown of ITGB2 expression inhibited the proliferation of glioma cells in vitro and induced cell cycle arrest. E2F2 bound to the promoter region of ITGB2. Overexpression of E2F2 promoted the expression of ITGB2 protein, and knockdown of E2F2 inhibited the expression of ITGB2 protein. Overexpression of E2F2 reversed the inhibitory effect of knockdown of ITGB2 on the growth of glioma cells. Conclusion ITGB2 is highly expressed in glioma tissues and promotes glioma cell proliferation, and its high expression is regulated by E2F2.

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备注/Memo

备注/Memo:
（2023-07-18收稿，2024-04-04修回）
基金项目：武汉市知识创新专项曙光计划项目（2022020801020483）
通信作者：邓　钢，Email：gang.deng@whu.edu.cn
更新日期/Last Update: 2024-08-30