[1]王昆鹏,张建党,董孟宁,等.lncRNA SNHG7对胶质瘤细胞侵袭、迁移的影响[J].中国临床神经外科杂志,2022,27(08):662-666.[doi:10.13798/j.issn.1009-153X.2022.08.012]
　WANG Kun-peng,ZHANG Jian-dang,DONG Meng-ning,et al.[J].,2022,27(08):662-666.[doi:10.13798/j.issn.1009-153X.2022.08.012]
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lncRNA SNHG7对胶质瘤细胞侵袭、迁移的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
27
期数:
2022年08期
页码:
662-666
栏目:
实验研究
出版日期:
2022-08-31

文章信息/Info

文章编号:
1009-153X(2022)08-0662-05
作者:
王昆鹏; 张建党; 董孟宁; 刘素杰; 徐广建
473000 河南，南阳市中心医院神经外科（王昆鹏、张建党、董孟宁、刘素杰、徐广建）
Author(s):
WANG Kun-peng;  ZHANG Jian-dang;  DONG Meng-ning;  LIU Su-jie;  XU Guang-jian
Department of Neurosurgery, Nanyang Central Hospital, Nanyang 473000, China
关键词:
胶质瘤; U87细胞; 长链非编码RNA; 小核仁RNA宿主基因7（SNHG7）; miR-4516; 生存预后; 细胞侵袭; 细胞迁移
Keywords:
Glioma;  miR-9-3p;  Long non-coding RNA (lncRNA);  Small nucleolar RNA host gene 7 (SNHG7);  Survival prognosis;  Cell invasion;  Cell migration
分类号:
R739.41
DOI:
10.13798/j.issn.1009-153X.2022.08.012
文献标志码:
A
摘要:
目的 探讨长链非编码RNA（lncRNA）小核仁RNA宿主基因7（SNHG7）与胶质瘤生存预后的关系，及其对胶质瘤细胞侵袭和迁移的影响。方法 选取2015年6月至2016年3月手术切除的胶质瘤组织80例（术后随访截止2020年4月）和50例颅脑损伤颅内减压术中切取的非肿瘤脑组织为对照，用RT-PCR法检测lncRNA SNHG7的表达水平。体外培养胶质瘤U87细胞，转染不同质粒敲低lncRNA SNHG7表达，用Transwell实验检测细胞侵袭和迁移能力；双荧光素酶报告基因实验检测lncRNA SNHG7对miR-4516的调控作用。结果 胶质瘤组织lncRNA SNHG7表达量明显高于对照组（P<0.05）；多因素Cox回归分析显示，lncRNA SNHG7高表达是胶质瘤病人不良生存预后的独立影响因素（P<0.05）；生存曲线分析显示，高表达组胶质瘤病人中位总生存期较低表达组明显缩短（P<0.05）。敲低lncRNA SNHG7表达，明显抑制U87细胞侵袭和迁移力（P<0.05）。双荧光素酶报告基因实验证实lncRNA SNHG7靶向上调miR-4516表达，上调miR-4516可逆转敲低lncRNA SNHG7表达对胶质瘤U87细胞侵袭和迁移能力的作用（P<0.05）。结论 胶质瘤组织lncRNA SNHG7呈高表达，与病人不良生存预后有关。lncRNA SNHG7通过靶向调控上调miR-4516表达促进胶质瘤细胞的侵袭和迁移。
Abstract:
Objective To investigate the relationship between long non-coding RNA (lncRNA) small nucleolar RNA host gene 7 (SNHG7) and survival prognosis of glioma patients, and its effect on glioma cell invasion and migration. Methods The expression levels of lncRNA SNHG7 were detected by RT-PCR in glioma tissues obtained from 80 glioma patients who underwent surgery from June 2015 to March 2016 (postoperative follow-up ended in April 2020) and in non-tumor cerebral tissues obtained from 50 patients with traumatic brain injury (control group) during decompression. Glioma U87 cells were cultured in vitro, and the cells were transfected with different plasmids to knock down the expression of lncRNA SNHG7, and the ability of cell invasion and migration was detected by Transwell assay. The regulation of lncRNA SNHG7 on miR-4516 was verified by dual-luciferase reporter gene assay. Results The expression level of lncRNA SNHG7 in glioma tissues was significantly higher than that in the control group (P<0.05). Multivariate Cox regression analysis showed that the high expression of lncRNA SNHG7 was an independent risk factor for poor survival prognosis of glioma patients (P<0.05). Survival curve analysis showed that the median overall survival of glioma patients in the high expression group was significantly shorter than that in the low expression group (P<0.05). Knocking down the expression of lncRNA SNHG7 significantly inhibited the invasion and migration of U87 cells (P<0.05). The dual-luciferase reporter gene assay confirmed that lncRNA SNHG7 up-regulated miR-4516 expression, and up-regulation of miR-4516 could reverse the effect of knockdown of lncRNA SNHG7 expression on the invasion and migration of glioma U87 cells (P<0.05). Conclusions The high expression of lncRNA SNHG7 in glioma tissue is associated with poor survival prognosis of glioma patients. The lncRNA SNHG7 promotes the invasion and migration of glioma cells by up-regulation of miR-4516 expression.

参考文献/References:

[1]Cao M, Li H, Sun D, et al. Cancer burden of major cancers in China: a need for sustainable actions [J]. Cancer Commun (Lond), 2020, 40(5): 205-210.
[2]Sledzińska P, Bebyn MG, Furtak J, et al. Prognostic and predictive biomarkers in gliomas [J]. Int J Mol Sci, 2021, 22(19): 10373.
[3]McCabe EM, Rasmussen TP. lncRNA involvement in cancer stem cell function and epithelial-mesenchymal transitions [J]. Semin Cancer Biol, 2021, 75: 38-48.
[4]Winkle M, El-Daly SM, Fabbri M, et al. Noncoding RNA therapeutics--challenges and potential solutions [J]. Nat Rev Drug Discov, 2021, 20(8): 629-651.
[5]Zheng J, Zhou Z, Qiu Y, et al. A prognostic ferroptosis-related lncRNAs signature associated with immune land-scape and radiotherapy response in glioma [J]. Front Cell Dev Biol, 2021, 9: 675555.
[6]Venkatesh J, Wasson MD, Brown JM, et al. LncRNA-miRNA axes in breast cancer: novel points of interaction for strategic attack [J]. Cancer Lett, 2021, 509: 81-88.
[7]佟丽斐，张　霞，杨玉蝶，等. 敲低lncRNA SNHG7通过抑制ROCK1降低前列腺癌细胞增殖和迁移能力[J]. 中国医科大学学报，2022，51（4）：301-305.
[8]Cheng D, Fan J, Qin K, et al. LncRNA SNHG7 regulates mesenchymal stem cell through the Notch1/Jagged1/Hes-1 signaling pathway and influences folfirinox resistance in pancreatic cancer [J]. Front Oncol, 2021, 11: 719855.
[9]Peng Z, Liu C, Wu M. New insights into long noncoding RNAs and their roles in glioma [J]. Mol Cancer, 2018, 17(1): 61.
[10]Gao YT, Zhou YC. Long non-coding RNA (lncRNA) small nucleolar RNA host gene 7 (SNHG7) promotes breast cancer progression by sponging miRNA-381 [J]. Eur Rev Med Pharmacol Sci, 2019, 23(15): 6588-6595.
[11]She K, Yan H, Huang J, et al. miR-193b availability is antagonized by LncRNA-SNHG7 for FAIM2-induced tumour progression in non-small cell lung cancer [J]. Cell Prolif, 2018, 51(1): e12406.
[12]Cheng G, Zheng J, Wang L. LncRNA SNHG7 promotes glioma cells viability, migration and invasion by regulating miR-342-3p/AKT2 axis [J]. Int J Neurosci, 2021, 131(12): 1190-1202.
[13]Deng Y, Cheng L, Lv Z, et al. lncRNA SNHG7 promotes cell proliferation in glioma by acting as a competing endo-genous RNA and sponging miR-138-5p to regulate EZH2 expression [J]. Oncol Lett, 2021, 22(1): 565.
[14]Ren J, Yang Y, Xue J, et al. Long noncoding RNA SNHG7 promotes the progression and growth of glioblastoma via inhibition of miR-5095 [J]. Biochem Biophys Res Commun, 2018, 496(2): 712-718.
[15]Li H, Li T, Huang D, et al. Long noncoding RNA SNHG17 induced by YY1 facilitates the glioma progression through targeting miR-506-3p/CTNNB1 axis to activate Wnt/β-catenin signaling pathway [J]. Cancer Cell Int, 2020, 20: 29.
[16]Chen Y, Yuan S, Ning T, et al. SNHG7 facilitates glioblas-toma progression by functioning as a molecular sponge for microRNA-449b-5p and thereby increasing MYCN expression [J]. Technol Cancer Res Treat, 2020, 19: 1533033820945802.
[17]兰　展，仇　洪，冯　磊，等. 人脑胶质母细胞瘤miR-4516表达与病人预后的关系[J]. 中国临床神经外科杂志，2022，27（2）：107-109.
[18]Li Q, Wang W, Yang T, et al. LINC00520 up-regulates SOX5 to promote cell proliferation and invasion by miR-4516 in human hepatocellular carcinoma [J]. Biol Chem, 2022, 13(1): 122-127.

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　PENG Ze-sheng,TIAN Dao-feng,ZHANG Shen-qi,et al.Effects of MircoRNA-370-3p on proliferation of glioma cell line U87-MG and its potential mechanism[J].,2016,(08):223.[doi:10.13798/j.issn.1009-153X.2016.04.010]
[10]冯　驰　郭双毅　程龙海　罗　杰.氯喹通过抑制自噬促进TRAIL诱导的胶质瘤细胞凋亡[J].中国临床神经外科杂志,2016,(04):227.[doi:10.13798/j.issn.1009-153X.2016.04.011]
　FENG Chi,GUO Shuang-yi,CHENG Long-hai,et al.Chloroquine promotes TRAIL-induced apoptosis of glioma cells through inhibition of autophagy[J].,2016,(08):227.[doi:10.13798/j.issn.1009-153X.2016.04.011]
[11]胡　森　张　慧　步星耀　冯淼淼　王帮庆　王君毅　杨　斌　杨华超.白藜芦醇对裸鼠胶质瘤模型肿瘤生长的抑制作用[J].中国临床神经外科杂志,2019,(09):543.[doi:10.13798/j.issn.1009-153X.2019.09.010]
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[12]赵海康　潘　力　张　亮　冯小云　刘建荣　蒋小兵　高文文　马廉亭.替莫唑胺通过TFRC抑制U87胶质瘤细胞增殖和侵袭[J].中国临床神经外科杂志,2019,(11):685.[doi:10.13798/j.issn.1009-153X.2019.11.015]
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　LI Ji-qiang,DUAN Fa-liang,WU Jing-lei,et al.Effect of down-regulation of BAG3 on proliferation and apoptosis of glioma U87 cells[J].,2022,27(08):913.[doi:10.13798/j.issn.1009-153X.2022.11.010]
[18]热斯江·居马色衣提,张丽娜,秦虎,等.下调RNA甲基化酶NSUN2表达对脑胶质瘤U87细胞增殖、侵袭、迁移的影响[J].中国临床神经外科杂志,2023,28(02):102.[doi:10.13798/j.issn.1009-153X.2023.02.012]
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备注/Memo

备注/Memo:
（2022-06-16收稿，2022-07-25修回）
通讯作者：张建党，E-mail：wangkunpeng129@163.com
更新日期/Last Update: 2022-09-30