[1]丁萌,王红娟,周洁,等.lncRNA ZNF674-AS1过表达对胶质瘤U87细胞增殖、侵袭和迁移的影响[J].中国临床神经外科杂志,2022,27(10):837-840847.[doi:10.13798/j.issn.1009-153X.2022.10.012]
　DING Meng,WANG Hong-juan,Zhou Jie,et al.Effects of over-expression of lncRNA ZNF674-AS1 on proliferation, invasion and migration of U87 glioma cells[J].,2022,27(10):837-840847.[doi:10.13798/j.issn.1009-153X.2022.10.012]
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lncRNA ZNF674-AS1过表达对胶质瘤U87细胞增殖、侵袭和迁移的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
27
期数:
2022年10期
页码:
837-840847
栏目:
实验研究
出版日期:
2022-10-31

文章信息/Info

Title:
Effects of over-expression of lncRNA ZNF674-AS1 on proliferation, invasion and migration of U87 glioma cells
文章编号:
1009-153X（2022）10-0837-04
作者:
丁萌; 王红娟; 周洁; 高强
266042山东青岛，青岛大学附属青岛市中心医院神经外科（丁萌、王红娟、周洁、高强）
Author(s):
DING Meng;  WANG Hong-juan;  Zhou Jie;  GAO Qiang
Department of Neurosurgery, Qingdao Central Hospital Affiliated to Qingdao University, Qingdao 266042, China
关键词:
胶质瘤; 长链非编码RNA; RNAZNF674-AS1; 细胞增殖; 细胞侵袭; 细胞迁移
Keywords:
Glioma;  long non-coding RNA;  ZNF674-AS1;  Cell proliferation;  Cell invasion;  Cell migration;  SOX9
分类号:
R739.41
DOI:
10.13798/j.issn.1009-153X.2022.10.012
文献标志码:
A
摘要:
目的 探讨长链非编码RNA（lncRNA）ZNF674-AS1过表达对胶质细胞瘤增殖、侵袭、迁移的影响。方法 体外培养正常星形胶质细胞（HA1800）和胶质瘤细胞（A172、U251、U87、U373），RT-PCR检测lncRNA ZNF674-AS1表达水平。将ZNF674-AS1 mimics转染U87细胞上调ZNF674-AS1表达，以转染阴性对照序列为对照，CCK8法检测细胞增殖能力，Transwell实验检测细胞侵袭和迁移能力。Starbase软件预测lncRNA ZNF674-AS1靶基因并应用双荧光素酶报告基因实验验证。结果 与正常星形胶质细胞（HA1800）比较，胶质瘤细胞（A172、U251、U87、U373）lncRNA ZNF674-AS1的表达水平均明显降低（P<0.05），其中U87细胞表达水平最低。上调U87细胞lncRNA ZNF674-AS1表达，明显抑制U87细胞增殖、侵袭、迁移能力（P<0.05）。Starbase软件预测显示lncRNA ZNF674-AS1与性别决定区 Y 框蛋白 9（SOX9）基因有结合位点，双荧光素酶报告基因实验结果显示，SOX9基因是lncRNA ZNF674-AS1靶基因。上调U87细胞lncRNA ZNF674-AS1表达的同时沉默SOX9基因表达，明显增强U87细胞增殖、侵袭和迁移能力（P<0.05）。结论 胶质瘤lncRNA ZNF674-AS1呈低表达，可能通过靶向下调SOX9基因表达，促进胶质瘤细胞增殖、侵袭和迁移。
Abstract:
Objective To investigate the effect of over-expression of lncRNA ZNF674-AS1 on proliferation, invasion and migration of U87 glioma cells. Methods The expression levels of lncRNA ZNF674-AS1 were detected by RT-PCR in normal astrocytes (HA1800) and glioma cells (A172, U251, U87, U373). ZNF674-AS1 mimics were transfected into U87 cells to up-regulate expression of lncRNA ZNF674-AS1, with negative sequence as control. Cell proliferation was detected by CCK8 assay, and cell invasion and migration were detected by Transwell assay. lncRNA ZNF674-AS1 target genes were predicted by Starbase software and verified by dual luciferase reporter gene assay. Results Compared with normal astrocytes (HA1800), the expression levels of lncRNA ZNF674-AS1 in glioma cells (A172, U251, U87, and U373) were significantly decreased (P<0.05), and the expression level of lncRNA ZNF674-AS1 in U87 cells was the lowest. Over-expression of lncRNA ZNF674-AS1 significantly inhibited the proliferation, invasion and migration of U87 cells (P<0.05). Starbase software predicted that lncRNA ZNF674-AS1 had a binding site with the sex determining region Y-box protein 9 (SOX9) gene, and the double luciferase reporter gene experiment showed that SOX9 gene was the target gene of lncRNA ZNF674-AS1. Over-expression of lncRNA ZNF674-AS1 combined with silence expression of SOX9 significantly enhanced the proliferation, invasion and migration of U87 cells (P<0.05). Conclusions The expression of lncRNA ZNF674-AS1 is down-regulated in glioma, which may promote the proliferation, invasion and migration of glioma cells by down-regulating expression of SOX9 gene.

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备注/Memo

备注/Memo:
（2022-07-27收稿，2022-08-26修回）
通讯作者：高　强，E-mail：gaoqiang0139@sina.com
更新日期/Last Update: 2022-11-30