[1]包逸雯,朱伟良.IDH突变调控BMP2、COL27A1和SERPINA5基因表达影响人脑胶质瘤的生存预后[J].中国临床神经外科杂志,2023,28(03):179-183.[doi:10.13798/j.issn.1009-153X.2023.03.011]
　BAO Yi-wen,ZHU Wei-liang.IDH mutation regulates expressions of BMP2, COL27A1, and SERPINA5 to improve survival prognoses of glioma patients[J].,2023,28(03):179-183.[doi:10.13798/j.issn.1009-153X.2023.03.011]
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IDH突变调控BMP2、COL27A1和SERPINA5基因表达影响人脑胶质瘤的生存预后()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
28
期数:
2023年03期
页码:
179-183
栏目:
论著
出版日期:
2023-03-31

文章信息/Info

Title:
IDH mutation regulates expressions of BMP2, COL27A1, and SERPINA5 to improve survival prognoses of glioma patients
文章编号:
1009-153X（2023）03-0179-05
作者:
包逸雯; 朱伟良
510220广州，南方医科大学珠江医院肿瘤科（包逸雯、朱伟良）
Author(s):
BAO Yi-wen;  ZHU Wei-liang
Department of Oncology, Zhujiang Hospital, Southern Medical University, Guangzhou 510220, China
关键词:
胶质瘤;  IDH突变; 生存预后; 基因表达
Keywords:
Gliomas;  IDH mutations;  Gene expression;  Survival prognosis
分类号:
A
DOI:
10.13798/j.issn.1009-153X.2023.03.011
文献标志码:
R739.41;Q786
摘要:
目的 探讨异柠檬酸脱氢酶（IDH）突变影响胶质瘤临床预后的潜在分子机制。方法 计算机检索CGGA和TCGA数据库，获取胶质瘤RNA测序数据（RNA-seq），应用生物信息学分析方法分析IDH突变改善胶质瘤临床预后的潜在分子机制。结果 从TCGA数据库下载胶质母细胞瘤（GBM）和低级别胶质瘤（LGG）相关RNA-seq，从CGCA数据量下载648例胶质瘤的RNA-seq（234 例GBM和414例LGG），从GEO数据量下载单细胞RNA-seq数据GSE131928数据集（28例IDH野生型GBM）。GO和KEGG分析显示，IDH突变显著下调胞外基质（ECM）相关基因表达，GEPIA分析显示BMP2、COL27A1、SERPINA5、VEGFA基因表达水平与胶质瘤生存预后有关，多因素Cox比例回归风险模型分析显示BMP2、COL27A1、SERPINA5基因表达是胶质瘤生存预后独立影响因素，BMP2联合SERPINA5预测胶质瘤生存预后效果最好。结论 我们结果提示胶质瘤IDH基因突变，可能通过调控BMP2、COL27A1和SERPINA5基因表达，影响病人生存预后。
Abstract:
Objective To explore the potential molecular mechanisms of isocitrate dehydrogenase (IDH) mutation improving the clinical prognoses of glioma patients. Methods Glioma RNA sequencing data (RNA-seq) were obtained from CGGA and TCGA databases. The potential molecular mechanisms of IDH mutation to improve the clinical prognoses of glioma patients was analyzed using bioinformatics methods. Results RNA-seq related to glioblastoma (GBM) and low-grade glioma (LGG) were downloaded from the TCGA database, and RNA-seq of 648 gliomas (234 GBM and 414 LGG) were downloaded from CGCA data. Single cell RNA-seq dataset GSE131928 (28 IDH wild-type GBM) was downloaded from GEO database. GO and KEGG analyses showed that IDH mutation significantly down-regulated the expression of extracellular matrix related genes. GEPIA analysis showed that the expression levels of BMP2, COL27A1, SERPINA5 and VEGFA genes were correlated with the survival prognoses of glioma patients. Multivariate Cox proportional regression risk model analysis showed that expressions of BMP2, COL27A1, and SERPINA5 genes were independent risk factors for survival prognoses of glioma patients, and BMP2 combined with SERPINA5 was the best predictor for survival prognoses of glioma patients. Conclusions Our results suggest that IDH gene mutation may improve survival prognoses of glioma patients by regulating the expressions of BMP2, COL27A1, and SERPINA5.

参考文献/References:

[1]Poon MTC, Brennan PM, Jin K, et al. Might changes in diagnostic practice explain increasing incidence of brain and central nervous system tumors: a population-based study in Wales (United Kingdom) and the United States [J]. Neuro Oncol, 2021, 23(6): 979-989.
[2]Ostrom QT, Cioffi G, Gittleman H, et al. CBTRUS statistical report: primary brain and other central nervous system tumors diagnosed in the United States in 2012-2016 [J]. Neuro Oncol, 2019, 21(Suppl 5): v1-v100.
[3]Tykocki T, Eltayeb M. Ten-year survival in glioblastoma: a systematic review [J]. J Clin Neurosci, 2018, 54: 7-13.
[4]Ostrom QT, Cote DJ, Ascha M, et al. Adult glioma incidence and survival by race or ethnicity in the United States from 2000 to 2014 [J]. JAMA Oncol, 2018, 4(9): 1254-1262.
[5]Xia L, Wu B, Fu Z, et al. Prognostic role of IDH mutations in gliomas: a meta-analysis of 55 observational studies [J]. Oncotarget, 2015, 6(19): 17354-65.
[6]Sahai E, Astsaturov I, Cukierman E, et al. A framework for advancing our understanding of cancer-associated fibroblasts [J]. Nat Rev Cancer, 2020, 20(3): 174-186.
[7]Ducassou A, Uro-Coste E, Verrelle P, et al. αvβ3 integrin and fibroblast growth factor receptor 1 (FGFR1): prognostic factors in a phase I-II clinical trial associating continuous administration of Tipifarnib with radiotherapy for patients with newly diagnosed glioblastoma [J]. Eur J Cancer, 2013, 49(9): 2161-2169.
[8]Bahleda R, Italiano A, Hierro C, et al. Multicenter Phase I Study of Erdafitinib (JNJ-42756493), oral pan-fibroblast growth factor receptor inhibitor, in patients with advanced or refractory solid tumors [J]. Clin Cancer Res, 2019, 25(16): 4888-4897.
[9]Zhao Z, Zhang KN, Wang Q, et al. Chinese Glioma Genome Atlas (CGGA): a comprehensive resource with functional genomic data from Chinese glioma patients [J]. Genomics Proteomics Bioinformatics, 2021, 19(1): 1-12.
[10] Neftel C, Laffy J, Filbin MG, et al. An integrative model of cellular states, plasticity, and genetics for glioblastoma [J]. Cell, 2019, 178(4): 835-849.
[11] Stupp R, Mason WP, van den Bent MJ, et al. Radiotherapy plus concomitant and adjuvant temozolomide for glioblastoma [J]. N Engl J Med, 2005, 352(10): 987-996.
[12] Fukuya Y, Ikuta S, Maruyama T, et al. Tumor recurrence patterns after surgical resection of intracranial low-grade gliomas [J]. J Neurooncol, 2019, 144(3): 519-528.
[13] Yang X, Li D, Cheng S, et al. The correlation of bone morphogenetic protein 2 with poor prognosis in glioma patients [J]. Tumour Biol, 2014, 35(11): 11091-11095.
[14] Wang JJ, Wang H, Zhu BL, et al. Development of a prognostic model of glioma based on immune-related genes [J]. Oncol Lett, 2021, 21(2): 116.
[15] Zeng WJ, Yang YL, Wen ZP, et al. Identification of gene expression and DNA methylation of SERPINA5 and TIMP1 as novel prognostic markers in lower-grade gliomas [J]. PeerJ, 2020, 8: e9262.

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备注/Memo

备注/Memo:
（2022-10-10收稿，2023-02-13修回）
基金项目：广东省自然科学基金（2018A030313846；2021A1515012593）
通讯作者：朱伟良，E-mail：duarion@126.com
更新日期/Last Update: 2022-04-30