[1]鲁飞,柳保丽,张万宏,等.ITGA4基因在脑胶质瘤中的表达及临床意义[J].中国临床神经外科杂志,2023,28(03):184-187215.[doi:10.13798/j.issn.1009-153X.2023.03.012]
　LU Fei,LIU Bao-li,ZHANG Wan-hong,et al.Expression of integrin α4 gene in human brain gliomas and its clinical significance based on bioinformatics analysis[J].,2023,28(03):184-187215.[doi:10.13798/j.issn.1009-153X.2023.03.012]
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ITGA4基因在脑胶质瘤中的表达及临床意义()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
28
期数:
2023年03期
页码:
184-187215
栏目:
论著
出版日期:
2023-03-31

文章信息/Info

Title:
Expression of integrin α4 gene in human brain gliomas and its clinical significance based on bioinformatics analysis
文章编号:
1009-153X（2023）03-0184-04
作者:
鲁飞; 柳保丽; 张万宏; 吴恒浩; 张圣旭
453003河南新乡，新乡医学院（鲁飞、柳保丽、张万宏）；475000河南，开封市中心医院神经外科（鲁飞、张万宏、吴恒浩、张圣旭）；453002河南新乡，新乡医学院第二附属医院神经电生理科（柳保丽）
Author(s):
LU Fei1; 2;  LIU Bao-li1; 3;  ZHANG Wan-hong1; 2;  WU Heng-hao2;  ZHANG Sheng-xu2
1.Xinxiang Medical University, Xinxiang 453003, China; 2.Department of Neurosurgery, Kaifeng Central Hospital, Kaifeng 475000, China; 3.Department of Neuroelectrobiology, The Second Affiliated Hospital of Xinxiang Medical University, Xinxiang 453002, China
关键词:
胶质瘤; 整合素α4（ITGA4）; 基因表达; 生信分析
Keywords:
Glioma;  Integrin α4 (ITGA4);  Gene expression;  Bioinformatics analysis
分类号:
A
DOI:
10.13798/j.issn.1009-153X.2023.03.012
文献标志码:
R739.41;Q786
摘要:
目的 探讨整合素α4（ITGA4）基因在脑胶质瘤中的表达与临床意义。方法 计算机检索CGGA数据库获取mRNAseq-325数据集中325例脑胶质瘤的转录组测序（RNA-seq）数据及临床资料，并用mRNAseq-693数据集中693例脑胶质瘤进行验证。结果 胶质瘤ITGA4基因呈高表达（P<0.05），与胶质瘤IDH基因型、1p/19q共缺失状态、WHO病理分级有关（P<0.05），与胶质瘤MGMT启动子甲基化状态无明显关系（P>0.05）。多因素Cox回归分析显示，ITGA4高表达是胶质瘤生存预后不良的独立危险因素（P<0.05）。生存曲线分析显示，ITGA4高表达胶质瘤病人生存期明显缩短（P<0.05）。GO分析和KEGG分析显示，ITGA4基因调控的生物过程包括细胞粘附、血管生成及细胞迁移等，主要调控PI3K/AKT信号通路。结论 我们的结果提示胶质瘤ITGA4呈高表达，与胶质瘤不良生存预后密切相关，主要调控PI3K/AKT信号通路。
Abstract:
Objective To investigate the expression of integrin α4 (ITGA4) gene in human brain gliomas and its clinical significance. Methods The transcriptional sequencing (RNA-seq) data and clinical data of 325 patients with gliomas in mRNAseq-325 dataset were downloaded from CGGA database, and 693 patients with gliomas in mRNAseq-693 dataset were used to verified. Results ITGA4 gene was highly expressed in human brain gliomas (P<0.05), which was related to IDH genotype, 1p/19q codeletion status, and WHO pathological grade (P<0.05), and was not relatted to MGMT promoter methylation status (P>0.05). Multivariate Cox regression analysis showed that high expression of ITGA4 was an independent risk factor for poor survival prognosis of glioma patients (P<0.05), and survival curve analysis showed that the survival time of glioma patients with high ITGA4 expression was significantly shortened (P<0.05). GO and KEGG analyses showed that ITGA4 gene were involved in biological processes including cell adhesion, angiogenesis, and cell migration, and mainly involved in the PI3K/AKT signaling pathway. Conclusions Our results suggest that ITGA4 is highly expressed in gliomas, which is closely related to the poor survival prognoses of glioma patients. ITGA4 mainly regulates the PI3K/AKT signaling pathway.

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备注/Memo

备注/Memo:
（2022-11-08收稿，2023-02-07修回）
通讯作者：张万宏，E-mail：zhangwanhong2007@163.com
更新日期/Last Update: 2022-04-30