[1]李继强,段发亮,吴京雷,等.下调BAG3表达对胶质瘤U87细胞增殖和凋亡的影响[J].中国临床神经外科杂志,2022,27(11):913-916.[doi:10.13798/j.issn.1009-153X.2022.11.010]
　LI Ji-qiang,DUAN Fa-liang,WU Jing-lei,et al.Effect of down-regulation of BAG3 on proliferation and apoptosis of glioma U87 cells[J].,2022,27(11):913-916.[doi:10.13798/j.issn.1009-153X.2022.11.010]
点击复制

下调BAG3表达对胶质瘤U87细胞增殖和凋亡的影响()
分享到：

《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
27
期数:
2022年11期
页码:
913-916
栏目:
实验研究
出版日期:
2022-11-30

文章信息/Info

Title:
Effect of down-regulation of BAG3 on proliferation and apoptosis of glioma U87 cells
文章编号:
1009-153X（2022）11-0913-04
作者:
李继强; 段发亮; 吴京雷; 陈晓斌; 张严国; 丁伟
430022，武汉市第一医院神经外科（李继强、段发亮、吴京雷、陈晓斌、张严国、丁伟）
Author(s):
LI Ji-qiang;  DUAN Fa-liang;  WU Jing-lei;  CHEN Xiao-bin;  ZHANG Yan-guo;  DING Wei
Department of Neurosurgery, Wuhan NO. 1 Hospital, Wuhan 430022, China
关键词:
胶质瘤; U87细胞; Bcl2相关性抗凋亡基因3; 细胞增殖; 细胞凋亡
Keywords:
Glioma;  U87 cells;  Bcl2-associated athanogene 3 (BAG3);  Cell proliferation;  Cell apoptosis
分类号:
R739.41
DOI:
10.13798/j.issn.1009-153X.2022.11.010
文献标志码:
A
摘要:
目的 探讨下调BAG3表达对胶质瘤U87细胞增殖和凋亡的影响。方法 体外培养U87细胞，转染pEGFP-BAG3-shRNA质粒构建BAG3低表达胶质瘤U87细胞株（低表达组），转染pEGFP-shRNA对照质粒为对照组；利用RT-PCR和免疫印迹法鉴定；利用CCK8和EdU检测U87细胞增殖，利用流式细胞术检测U87细胞凋亡。结果 与对照组相比，RT-PCR和免疫印迹法检测结果显示低表达组BAG3 mRNA和蛋白表达水平均明显降低（P<0.05），低表达组24、48、72 h细胞增殖水平均明显降低（P<0.05），低表达组细胞凋亡率明显增高（P<0.05）。结论 下调胶质瘤U87细胞BAG3表达显著抑制其增殖、促进其凋亡。
Abstract:
Objective To explore the effect of down-regulation of BAG3 on the proliferation and apoptosis of glioma U87 cells. Methods U87 cells with down-regulation of BAG3 were reconstruted by transfection of shBAG3 plasmids, and were identified by the RT-PCR and western blotting. The proliferation of U87 cells was tested by CCK8 and EdU assay, and the apoptosis of U87 cells was tested by flow cytometry. Results The results of RT-PCR and western blotting showed that mRNA and protein expression levels of BAG3 were significantly down-regulated after transfection of shBAG3 plasmids. After transfection of shBAG3 plasmids, CCK8 and EdU assay showed that the proliferation of U87 cells was significantly inhibited and flow cytometry showed that the apoptosis of U87 cells was significantly promoted. Conclusions Down-regulation of BAG3 inhibits proliferation and promotes apoptosis of glioma U87 cells.

参考文献/References:

[1] 胡峻龙，赵世光. 替莫唑胺治疗脑胶质瘤的新进展[J]. 中国临床神经外科杂志，2021，26（8）：647-649.
[2] 赵海康，潘　力，张　亮，等. 替莫唑胺通过TFRC抑制U87胶质瘤细胞增殖和侵袭[J]. 中国临床神经外科杂志，2019，24（11）：685-689.
[3] 刘艳霞，李雨浓，傅若秋，等. BAG3的结构和功能的研究进展[J]. 生命科学，2017，29（11）：1133-1140.
[4] 刘宝辉，孙　前，袁凡恩，等. 高级别胶质瘤的治疗进展[J]. 中国临床神经外科杂志，2021，26（5）：380-384.
[5] Sherman MY, Gabai V. The role of Bag3 in cell signaling [J]. J Cell Biochem, 2022, 123(1): 43-53.
[6] Antonietti P, Linder B, Hehlgans S, et al. Interference with the HSF1/HSP70/BAG3 pathway primes glioma cells to matrix detachment and BH3 mimetic-induced apoptosis [J]. Mol Cancer Ther, 2017, 16(1): 156-168.
[7] Zhu H, Wu W, Fu Y, et al. Overexpressed BAG3 is a potential therapeutic target in chronic lymphocytic leukemia [J]. Ann Hematol, 2014, 93(3): 425-435.
[8] 胡　平，叶立果，陈谦学. 脑胶质瘤IDH基因突变的分子机制及其相关靶向治疗的研究进展[J]. 中国临床神经外科杂志，2022，27（5）：405-406.
[9] Roperto S. Role of BAG3 in bovine deltapapillomavirus-mediated autophagy [J]. J Cell Biochem, 2022, 123(1): 59-64.
[10] Linder B, Klein C, Hoffmann ME, et al. BAG3 is a negative regulator of ciliogenesis in glioblastoma and triple-negative breast cancer cells [J]. J Cell Biochem, 2022, 123: 77-90.
[11] Sherman MY, Gabai V. The role of BAG3 in cell signaling [J]. J Cell Biochem, 2022, 123(1): 43-53.
[12] De Marco M, Troisi J, Giugliano L, et al. BAG3 interacts with p53 in endometrial carcinoma [J]. Cell Oncol, 2020, 43(5): 957-960.
[13] Rocchi A, Wollebo HS, Khalili K. Rrotien quality control in glioblastoma: a review of the current literature with new perspectives on therapeutic targets [J]. Int J Mol Sci, 2022, 23(17): 9734.

相似文献/References:

[1]常英男　王　策　赵天书.SOX2基因在胶质瘤中的表达及生物学作用[J].中国临床神经外科杂志,2016,(05):270.[doi:10.13798/j.issn.1009-153X.2016.05.005]
　CHANG Ying-nan,WANG Ce,ZHAO Tian-shu..Expression of SOX2 gene and its biological role in human gliomas[J].,2016,(11):270.[doi:10.13798/j.issn.1009-153X.2016.05.005]
[2]赛　克　陈忠平.胶质瘤的疗效评价[J].中国临床神经外科杂志,2016,(06):321.[doi:10.13798/j.issn.1009-153X.2016.06.001]
[3]许　耿　路俊锋　杨　忠　施建斌　吴秋月　王　烨　庄冬晓　吴劲松.神经电生理监测技术在功能区胶质瘤术中的应用[J].中国临床神经外科杂志,2016,(06):323.[doi:10.13798/j.issn.1009-153X.2016.06.002]
　XU Geng,LU Jun-feng,YANG Zhong,et al.Application of intraoperative neuroelectrophysiologic monitoring to gliomas resection in eloquent function brain regions[J].,2016,(11):323.[doi:10.13798/j.issn.1009-153X.2016.06.002]
[4]李剑峰　陈银生　赛　克　张湘衡　柯　超　杨群英　牟永告　许海雄　陈忠平.173例胶质瘤预后的影响因素分析[J].中国临床神经外科杂志,2016,(06):327.[doi:10.13798/j.issn.1009-153X.2016.06.003]
　LI Jian-feng,CHEN Yin-sheng,SAI Ke,et al.Prognostic factors for gliomas: analysis of 173 cases[J].,2016,(11):327.[doi:10.13798/j.issn.1009-153X.2016.06.003]
[5]云德波　杨宇焦　张　逵　范润金　张　渊　杜贻庆.瘤周谷氨酸、天门冬氨酸水平与胶质瘤继发性 癫痫的相关性[J].中国临床神经外科杂志,2016,(06):331.[doi:10.13798/j.issn.1009-153X.2016.06.004]
　YUN De-bo,YANG Yu-Jiao,ZHANG Kui,et al.Relationship of levels of glutamate and aspartate in peritumorous tissues with seizures in patients with gliomas[J].,2016,(11):331.[doi:10.13798/j.issn.1009-153X.2016.06.004]
[6]黄书岚　成于思　王　辉　汪超甲.FAK siRNA重组质粒的构建及其对胶质瘤U251细胞 增殖及侵袭能力的抑制作用[J].中国临床神经外科杂志,2016,(06):336.[doi:10.13798/j.issn.1009-153X.2016.06.006]
　HUANG Shu-lan,CHENG Yu-si,WANG Hui,et al.Construction of siRNA recombinant plasmid targeting focal adhesion kinase gene and its effect on the proliferation and invasiveness of human glioma U251cells[J].,2016,(11):336.[doi:10.13798/j.issn.1009-153X.2016.06.006]
[7]林　雨　张　恺　李　帅　杨学军.磁共振数字化手术单元在指导胶质瘤治疗中的作用[J].中国临床神经外科杂志,2016,(06):345.[doi:10.13798/j.issn.1009-153X.2016.06.009]
[8]戴黎明　徐成仕　王泽芬　曹长军　李志强.VEGF单抗对低氧环境下C6胶质瘤细胞侵袭性的影响[J].中国临床神经外科杂志,2016,(04):219.[doi:10.13798/j.issn.1009-153X.2016.04.009]
　DAI Li-ming,XU Cheng-shi,WANG Ze-fen,et al.Effect of nonoclonal antibody of VEGF on C6 glioma cells invasiveness under hypoxia and the role of FAK/Pyk2[J].,2016,(11):219.[doi:10.13798/j.issn.1009-153X.2016.04.009]
[9]彭泽生　田道锋　张申起　陈谦学.miR-370-3p对胶质母细胞瘤U87-MG细胞株增殖能力的影响[J].中国临床神经外科杂志,2016,(04):223.[doi:10.13798/j.issn.1009-153X.2016.04.010]
　PENG Ze-sheng,TIAN Dao-feng,ZHANG Shen-qi,et al.Effects of MircoRNA-370-3p on proliferation of glioma cell line U87-MG and its potential mechanism[J].,2016,(11):223.[doi:10.13798/j.issn.1009-153X.2016.04.010]
[10]冯　驰　郭双毅　程龙海　罗　杰.氯喹通过抑制自噬促进TRAIL诱导的胶质瘤细胞凋亡[J].中国临床神经外科杂志,2016,(04):227.[doi:10.13798/j.issn.1009-153X.2016.04.011]
　FENG Chi,GUO Shuang-yi,CHENG Long-hai,et al.Chloroquine promotes TRAIL-induced apoptosis of glioma cells through inhibition of autophagy[J].,2016,(11):227.[doi:10.13798/j.issn.1009-153X.2016.04.011]
[11]胡　森　张　慧　步星耀　冯淼淼　王帮庆　王君毅　杨　斌　杨华超.白藜芦醇对裸鼠胶质瘤模型肿瘤生长的抑制作用[J].中国临床神经外科杂志,2019,(09):543.[doi:10.13798/j.issn.1009-153X.2019.09.010]
　HU Sen,ZHANG Hui,BU Xing-yao,et al.Resveratrol inhibits tumor growth in nude mice model of glioma[J].,2019,(11):543.[doi:10.13798/j.issn.1009-153X.2019.09.010]
[12]赵海康　潘　力　张　亮　冯小云　刘建荣　蒋小兵　高文文　马廉亭.替莫唑胺通过TFRC抑制U87胶质瘤细胞增殖和侵袭[J].中国临床神经外科杂志,2019,(11):685.[doi:10.13798/j.issn.1009-153X.2019.11.015]
　ZHAO Hai-kang,PAN Li,ZHANG Liang,et al.Temozolomide inhibits proliferation and invasion of glioma U87 cells by down-regulation of TFRC[J].,2019,(11):685.[doi:10.13798/j.issn.1009-153X.2019.11.015]
[13]任光辉　李武雄　齐利豪.胶质瘤干细胞外泌体通过PI3K/Akt信号通路促进胶质瘤U87细胞侵袭、迁移[J].中国临床神经外科杂志,2021,26(03):176.[doi:10.13798/j.issn.1009-153X.2021.03.011]
　REN Guang-hui,LI Wu-xiong,QI Li-hao.Glioma stem cell-derived exosomes promote glioma U87 cell invasion and migration through PI3K/Akt signaling pathway[J].,2021,26(11):176.[doi:10.13798/j.issn.1009-153X.2021.03.011]
[14]潘　轲　向春晖　周　龙　汪　逵　王国堰.FOXC2过表达通过激活Wnt/β-catenin信号通路促进胶质瘤U87细胞增殖、迁移[J].中国临床神经外科杂志,2021,26(04):266.[doi:10.13798/j.issn.1009-153X.2021.04.013]
　PAN Ke,XIANG Chun-hui,ZHOU Long,et al.FOXC2 overexpression promotes proliferation, invasion and migration of glioma U87 cells via activation of Wnt/β-catenin signaling pathway[J].,2021,26(11):266.[doi:10.13798/j.issn.1009-153X.2021.04.013]
[15]姜　羽　吕国伟　张文进等.槐定碱通过Wnt/β-catenin信号通路抑制人胶质瘤U87细胞迁移、侵袭[J].中国临床神经外科杂志,2021,26(10):789.[doi:10.13798/j.issn.1009-153X.2020.10.013]
　JIANG Yu,Lü Guo-wei,ZHANG Wen-jin,et al.Sophoridine inhibits migration and invasion of human glioma U87 cells by inhibting Wnt/β-catenin signaling pathway[J].,2021,26(11):789.[doi:10.13798/j.issn.1009-153X.2020.10.013]
[16]王昆鹏,张建党,董孟宁,等.lncRNA SNHG7对胶质瘤细胞侵袭、迁移的影响[J].中国临床神经外科杂志,2022,27(08):662.[doi:10.13798/j.issn.1009-153X.2022.08.012]
　WANG Kun-peng,ZHANG Jian-dang,DONG Meng-ning,et al.[J].,2022,27(11):662.[doi:10.13798/j.issn.1009-153X.2022.08.012]
[17]陶祥,张文斐,冀保卫,等.抑制SSRP1对胶质瘤细胞增殖、化疗敏感性的影响[J].中国临床神经外科杂志,2022,27(09):760.[doi:10.13798/j.issn.1009-153X.2022.09.013]
　TAO Xiang,ZHANG Wen-fei,JI Bao-wei,et al.Effect of inhibition of SSRP1 on glioma cells proliferation and chemosensitivity to temozolomide[J].,2022,27(11):760.[doi:10.13798/j.issn.1009-153X.2022.09.013]
[18]热斯江·居马色衣提,张丽娜,秦虎,等.下调RNA甲基化酶NSUN2表达对脑胶质瘤U87细胞增殖、侵袭、迁移的影响[J].中国临床神经外科杂志,2023,28(02):102.[doi:10.13798/j.issn.1009-153X.2023.02.012]
　RESIJIANG Jumaseyiti,ZHANG Li-na,QIN Hu,et al.Effects of down-regulated expression of RNA methylase NSUN2 on proliferation, invasion, and migration of glioma U87 cells[J].,2023,28(11):102.[doi:10.13798/j.issn.1009-153X.2023.02.012]

备注/Memo

备注/Memo:
（2021-11-19收稿，2022-07-31修回）
更新日期/Last Update: 2022-12-31