[1]姜　羽　吕国伟　张文进等.槐定碱通过Wnt/β-catenin信号通路抑制人胶质瘤U87细胞迁移、侵袭[J].中国临床神经外科杂志,2021,26(10):789-792.[doi:10.13798/j.issn.1009-153X.2020.10.013]
　JIANG Yu,Lü Guo-wei,ZHANG Wen-jin,et al.Sophoridine inhibits migration and invasion of human glioma U87 cells by inhibting Wnt/β-catenin signaling pathway[J].,2021,26(10):789-792.[doi:10.13798/j.issn.1009-153X.2020.10.013]
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槐定碱通过Wnt/β-catenin信号通路抑制人胶质瘤U87细胞迁移、侵袭()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
26
期数:
2021年10期
页码:
789-792
栏目:
实验研究
出版日期:
2021-10-25

文章信息/Info

Title:
Sophoridine inhibits migration and invasion of human glioma U87 cells by inhibting Wnt/β-catenin signaling pathway
文章编号:
1009-153X（2021）10-0789-04
作者:
姜　羽　吕国伟　张文进等
作者单位：450000 郑州，郑州大学附属郑州中心医院神经外科（姜　羽、吕国伟、张文进、张　辉）
Author(s):
JIANG Yu;  Lü Guo-wei;  ZHANG Wen-jin;  et al
Department of Neurosurgery, Zhengzhou Central Hospital, Zhengzhou University, Zhengzhou 450000, China
关键词:
胶质瘤; U87细胞; 细胞迁移; 细胞侵袭; 槐定碱; 上皮间质转化
Keywords:
Human glioma U87 cells;  Sophoridine;  Cell migration;  Cell invasion;  Epithelial-mesenchymal transition
分类号:
A
DOI:
10.13798/j.issn.1009-153X.2020.10.013
文献标志码:
R 739.41; Q 786
摘要:
目的 探讨槐定碱对人胶质瘤U87细胞迁移、侵袭能力的影响及其作用机制。方法 体外培养人胶质瘤U87细胞，加入槐定碱[0 mg/ml（对照组），1.0 mg/ml，2.0 mg/ml，3.0 mg/ml]共培养24 h，划痕实验检测细胞迁移能力，Transwell小室实验检测细胞侵袭能力，免疫印迹法检测细胞β-catenin、E-cadherin、Vimentin和MMP-9蛋白表达，RT-PCR检测细胞Vimentin、MMP-9 mRNA表达。结果 槐定碱明显抑制U87细胞迁移、侵袭能力（P<0.001），而且随浓度增加抑制作用明显增强（P<0.001）。槐定碱明显抑制U87细胞β-catenin蛋白、Vimentin和MMP-9 mRNA和蛋白表达（P<0.01），明显增强E-cadherin蛋白表达（P<0.01），而且随浓度增加抑制作用明显增强（P<0.01）。结论 槐定碱能抑制人胶质瘤U87细胞的迁移和侵袭，机制可能与抑制Wnt/β-catenin信号通路的活性，阻断细胞上皮间质转化过程。
Abstract:
Objective To investigate the effect of sophoridine on the migration and invasion of human glioma U87 cells and its possible mechanism. Methods Human glioma U87 cells were co-cultured with sophoridine [0 mg/ml (control), 1.0 mg/ml, 2.0 mg/ml, 3.0 mg/ml] for 24h. The cell migration and invasion abilities were detected by scrach test and transwell assay, respectively. The protein expression levels of β-catenin, E-cadherin vimentin and MMP-9 was detected by western blotting. The mRNA expression levels of vimentin and MMP-9 were detected by RT-PCR. Results Sophoridine significantly inhibited the migration and invasion of U87 cells (P<0.001), and the inhibitory effect was significantly enhanced with the increase in concentration of sophoridine (P<0.001). Sophoridine significantly inhibited β-catenin protein, Vimentin and MMP-9 mRNA and protein expression and significantly enhanced E-cadherin protein expression in U87 cells (P<0.01), and the inhibitory effect was significantly enhanced with increasing concentration of sophoridine (P<0.01). Conclusions Sophoridine can inhibit the migration and invasion of human glioma U87 cells, which may be by inhibiting the Wnt/β-catenin signaling pathway and blocking the process of epithelial-mesenchymal transition.

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备注/Memo

备注/Memo:
基金项目：河南省2016年科技发展计划（162102310215）
通讯作者：张　辉，E-mail：chy298a@163.com
更新日期/Last Update: 1900-01-01