[1]谈胤求　陈谦学　张红波　吴　鹏　李云涛　王栋良　袁凡恩.长链非编码RNA PVT1对人脑胶质瘤U251细胞增殖能力的影响[J].中国临床神经外科杂志,2016,(10):608-610.[doi:10.13798/j.issn.1009-153X.2016.10.013]
　TAN Yin-qiu,CHEN Qian-xue,ZHANG Hong-bo,et al.Effect of lncRNA-PVT1 on proliferation of glioma U251 cells[J].,2016,(10):608-610.[doi:10.13798/j.issn.1009-153X.2016.10.013]
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长链非编码RNA PVT1对人脑胶质瘤U251细胞增殖能力的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年10期
页码:
608-610
栏目:
论著
出版日期:
2016-10-24

文章信息/Info

Title:
Effect of lncRNA-PVT1 on proliferation of glioma U251 cells
文章编号:
1009-153X（2016）10-0608-03
作者:
谈胤求　陈谦学　张红波　吴　鹏　李云涛　王栋良　袁凡恩
430060 武汉，武汉大学人民医院神经外科
Author(s):
TAN Yin-qiu;  CHEN Qian-xue;  ZHANG Hong-bo;  WU Peng;  LI Yun-tao;  WANG Dong-liang;  YUAN Fan-en
Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, China
关键词:
胶质瘤; U251细胞; 细胞增殖; 长链非编码RNA; PVT1基因; 小干扰RNA技术
Keywords:
U251 cells;  Glioma;  LncRNA-PVT1;  Expression;  Proliferation
分类号:
R 743.41; Q 786
DOI:
10.13798/j.issn.1009-153X.2016.10.013
文献标志码:
A
摘要:
目的 探讨长链非编码RNA PVT1（lncRNA-PVT1）对人脑胶质瘤U251细胞增殖能力的影响。方法 体外培养胶质瘤细胞系U251细胞，分别转染PVT1 siRNA（PVT1组）和negative control siRNA（对照组）。PCR法检测lncRNA-PVT1及C-MYC mRNA表达水平，CCK-8法检测细胞增殖能力，Western blot检测C-MYC蛋白表达水平。结果 PVT1组lncRNA-PVT1 mRNA表达水平（0.27±0.08）较对照组（1.0±0.30）明显降低（P<0.05）；PVT1组C-MYC mRNA表达水平（0.87±0.19）与对照组（1.0±0.15）无明显差异（P>0.05）。转染siRNA后1、2、3 d，PVT1组U251细胞活力较对照组均明显降低（P<0.05）。PTV1组C-MYC蛋白表达水平（0.29±0.12）较对照组（0.85±0.27）明显降低（P<0.05）。结论 降低lncRNA-PVT1表达水平能够抑制U251细胞增殖，其机制可能与lncRNA-PVT1够影响C-MYC蛋白的表达水平有关。
Abstract:
Objective To explore the effect of long non-coding RNA PVT1 (lncRNA-PVT1) on the proliferation of glioma U251 cells. Methods The cultured glioma U251 cells were divided into 2 groups, i.e. experimental group in which PVT1 small interfere RNA (SiRNA) were tansfected into U251 cells and control group, in which negative control SiRNA was transfected into U251 cells. The expressions of lncRNA-PVT1 and C-MYC mRNA were detected by real-time quantitative PCR (RT-qPCR) in both the groups, in which the expressions of C-MYC protein were detected by Western blot. The proliferation of the U251 cells was determined by Cell Counting Kit-8 (CCK8) assay in two groups. Results The expression of lncRNA-PVT1 mRNA (0.27±0.08) was significantly lower in the experimental group than that (1.0±0.30) in the control group (P<0.05), but there is no significant difference in the expression of C-MYC mRNA between the two groups (P>0.05). The expression of C-MYC protein was significantly higher in control group than that in the experimental group (P<0.05). The cells viability was significantly lower in the experimental group than that in the control group 1, 2 and 3 days after the transfection (P<0.05). Conclusions Down-regulating the expression of lncRNA-PVT1 can decrease the expression of C-MYC protein in U251 cells and inhibit the proliferation of U251 cells.

参考文献/References:

[1] Derrien T, Johnson R, Bussotti G, et al. The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression [J]. Genome Res, 2012, 22(9): 1775-1789.
[2] Niland CN, Merry CR, Khalil AM. Emerging roles for long non-coding RNAs in cancer and neurological disorders [J]. Front Genet, 2012, 3: 25.
[3] Meyer KB, Maia AT, O'Reilly M, et al. A functional variant at a prostate cancer predisposition locus at 8q24 is asso- ciated with PVT1 expression [J]. PLoS Genet, 2011, 7(7): e1002165.
[4] Yang YR, Zang SZ, Zhong CL, et al. Increased expression of the lncRNA PVT1 promotes tumorigenesis in non-small cell lung cancer [J]. Int J Clin Exp Pathol, 2014, 7(10): 6929- 6935.
[5] Wang F, Yuan JH, Wang SB, et al. Oncofetal long non- coding RNA PVT1 promotes proliferation and stem cell-like property of hepatocellular carcinoma cells by stabilizing NOP2 [J]. Hepatology, 2014, 60(4): 1278-1290.
[6] Zhang XW, Bu P, Liu L, et al. Overexpression of long non- coding RNA PVT1 in gastric cancer cells promotes the development of multidrug resistance [J]. Biochem Biophys Res Commun, 2015, 462(3): 227-232.
[7] Colombo T, Farina L, Macino G, et al. PVT1: a rising star among oncogenic long noncoding RNAs [J]. Biomed Res Int, 2015, 2015: 304208.
[8] Tseng YY, Moriarity BS, Gong W, et al. PVT1 dependence in cancer with MYC copy-number increase [J]. Nature, 2014, 512(7512): 82-86.
[9] Faria MH, Goncalves BP, Do PR, et al. Expression of Ki-67, topoisomerase IIalpha and c-MYC in astrocytic tumors: correlation with the histopathological grade and proliferative status [J]. Neuropathology, 2006, 26(6): 519-527.
[10] Odia Y, Orr BA, Bell WR, et al. cMYC expression in infil- trating gliomas: associations with IDH1 mutations, clinico- pathologic features and outcome [J]. J Neurooncol, 2013, 115(2): 249-259.
[11] Yeh E, Cunningham M, Arnold H, et al. A signalling path- way controlling c-Myc degradation that impacts oncogenic transformation of human cells [J]. Nat Cell Biol, 2004, 6(4): 308-318.

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备注/Memo

备注/Memo:
通讯作者：陈谦学，E-mail：chenqx666@sohu.com
更新日期/Last Update: 2016-10-25