[1]李成林　丁　妍　于爱清　杨艳芳　李东升.CXCR4表达上调对U251细胞增殖、侵袭、迁移的影响[J].中国临床神经外科杂志,2016,(02):97-100.[doi:10.13798/j.issn.1009-153X.2016.02.013]
　LI Cheng-lin,DING Yan,YU Ai-qing,et al.Effects of up-regulation of CXCR4 on proliferation, invasion and migration of U251 cells[J].,2016,(02):97-100.[doi:10.13798/j.issn.1009-153X.2016.02.013]
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CXCR4表达上调对U251细胞增殖、侵袭、迁移的影响()
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《中国临床神经外科杂志》[ISSN:1009-153X/CN:42-1603/TN]

卷:
期数:
2016年02期
页码:
97-100
栏目:
论著
出版日期:
2016-02-25

文章信息/Info

Title:
Effects of up-regulation of CXCR4 on proliferation, invasion and migration of U251 cells
文章编号:
1009-153X（2016）02-0097-04
作者:
李成林　丁　妍　于爱清　杨艳芳　李东升
430071 武汉，武汉大学中南医院神经外科（李成林、于爱清、李东升）；442000 十堰，湖北医药学院附属太和医院神经外科（丁妍、杨艳芳）
通讯作者：李东升，E-mail：dsl1698@aliyun.com
Author(s):
LI Cheng-lin1;  DING Yan2;  YU Ai-qing1;  YANG Yan-fang2;  LI Dong-sheng1.
1. Department of Neurosurgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;
2. Department of Neurosurgery, Affiliated Taihe Hospital, Hubei Medicine College, Shiyan 442000, China
关键词:
胶质瘤; U251细胞; CXC趋化因子受体4; 细胞增殖; 细胞侵袭; 细胞迁移
Keywords:
U251 cells;  CXC chemokine receptor-4 (CXCR4);  Expression;  Up-regulation;  proliferation;  Migration;  Invasion
分类号:
R 739.41; Q 789
DOI:
10.13798/j.issn.1009-153X.2016.02.013
文献标志码:
A
摘要:
目的 探讨CXC趋化因子-4受体（CXCR4）表达上调对U251细胞增殖、侵袭及迁移能力的影响及其作用机制。方法 构建携带CXCR4基因的质粒，采用电转染法将携带CXCR4基因的质粒整合到胶质瘤U251细胞的基因组中，然后将细胞分为空白对照组、阴性对照组和CXCR4上调组，分别从mRNA水平和蛋白水平检测CXCR4及其他相关基因的表达；MTT试验检测细胞增殖能力的改变；划痕试验检测细胞的迁移能力；Transwell侵袭试验检测细胞侵袭性的变化。结果 与空白对照组和阴性对照组相比，CXCR4上调组U251细胞CXCR4在mRNA和蛋白水平上表达都增强，细胞增殖、侵袭和迁移能力均显著增强；而空白对照组和阴性对照组之间并无明显变化。结论 CXCR4在胶质瘤的增殖、侵袭、迁移行为中发挥着重要的作用，可以被视为胶质瘤治疗的靶向基因。
Abstract:
Objective To study the effects of CXC chemokine receptor-4 (CXCR4) expression up-regulation on U251 cells proliferation, invasion and migration abilities and its possible mechanism. Methods Plasmids carrying CXCR4 gene sequence were constructed and transfected into U251 cells by electroporation. The blank control group, negative control group and experimental group were designed. The expression of CXCR4 mRNA and protein and other related genes were detected in U251 cells. U251 cells proliferation ability was detected by MTT assay, their migration ability was detected by scratch assay and the changes in U251 cell invasion were detected by Transwell invasion assay. Results The levels of CXCR4 mRNA and protein and Nanog mRNA expression were significantly higher in the experimental group than those in the blank control group and the negative control group. The abilities of U251 cells proliferation, migration and invasion were significantly stronger in the experimental group than those in the other two groups (P<0.05). Conclusions It is suggested that CXCR4 plays an important role in the proliferation, migration and invasion of glioma cells, and it may be regarded as a target gene for the treatment of glioma.

参考文献/References:

[1] Kros JM, Mustafa DM, Dekker LJ, et al. Circulating glioma biomarkers [J]. Neuro Oncol, 2015, 17(3): 343-360.
[2] Altieri R, Agnoletti A, Quattrucci F, et al. Molecular biology of gliomas: present and future challenges [J]. Transl Med UniSa, 2014, 10: 29-37.
[3] Xie Q, Mittal S, Berens ME. Targeting adaptive glioblastoma: an overview of proliferation and invasion [J]. Neuro Oncol, 2014, 16(12): 1575-1584.
[4] Nomura W, Koseki T, Ohashi N, et al. Trivalent ligands for CXCR4 bearing polyproline linkers show specific recogni- tion for cells with increased CXCR4 expression [J]. Org Biomol Chem, 2015, 13(32): 8734-8739.
[5] Kim KH, Chung WS, Kim Y, et al. Transcriptomic analysis reveals wound healing of morus alba root extract by up-re- gulating keratin filament and CXCL12/CXCR4 signaling [J]. Phytother Res, 2015, 29(8): 1251-1258.
[6] Ehtesham M, Thompson RC. CXCR4-expressing glial pre- cursor cells demonstrate enhanced migratory tropism for glioma [J]. J Cancer Ther, 2012, 3(6): 1086-1091.
[7] Im KS, Graef AJ, Breen M, et al. Interactions between CXCR4 and CXCL12 promote cell migration and invasion of canine hemangiosarcoma [J]. Vet Comp Oncol, 2015. Epub ahead of print.
[8] Panditharatna E, Yaeger K, Kilburn LB, et al. Clinicopatho- logy of diffuse intrinsic pontine glioma and its redefined ge- nomic and epigenomic landscape [J]. Cancer Genet, 2015, 208(7-8): 367-373.
[9] Nakano I, Garnier D, Minata M, et al. Extracellular vesicles in the biology of brain tumour stem cells--implications for inter-cellular communication, therapy and biomarker deve- lopment [J]. Semin Cell Dev Biol, 2015, 40: 17-26.
[10] Bian XW, Yang SX, Chen JH, et al. Preferential expression of chemokine receptor CXCR4 by highly malignant human gliomas and its association with poor patient survival [J]. Neurosurgery, 2007, 61(3): 570-579.
[11] Safa AR, Saadatzadeh MR, Cohen-Gadol AA, et al. Emer- ging targets for glioblastoma stem cell therapy [J]. J Biomed Res, 2015, 20: 30.
[12] Jung MJ, Rho JK, Kim YM, et al. Upregulation of CXCR4 is functionally crucial for maintenance of stemness in drug- resistant non-small cell lung cancer cells [J]. Oncogene, 2013, 32(2): 209-221.
[13] Yao C, Li P, Song H, et al. CXCL12/CXCR4 axis upregu- lates twist to induce EMT in human glioblastoma [J]. Mol Neurobiol, 2015. Epub ahead of print.
[14] Gagliardi F, Narayanan A, Reni M, et al. The role of CXCR4 in highly malignant human gliomas biology: current know- ledge and future directions [J]. Glia, 2014, 62(7): 1015- 1023.
[15] Terasaki M, Sugita Y, Arakawa F, et al. CXCL12/CXCR4 signaling in malignant brain tumors: a potential pharmaco- logical therapeutic target [J]. Brain Tumor Pathol, 2011, 28 (2): 89-97.
[16] Trautmann F, Cojoc M, Kurth I, et al. CXCR4 as biomarq 2014, 90(8): 687-699.

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更新日期/Last Update: 2016-02-25